pancytokeratin panck (Novus Biologicals)

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Novus Biologicals pancytokeratin panck
Pancytokeratin Panck, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pancytokeratin/Cytokeratin%2C+pan+Antibody+(AE-1%2FAE-3)+-+Azide+and+BSA+Free/pm41430030-48-11-16
Average 95 stars, based on 47 article reviews

pancytokeratin panck - by Bioz Stars, 2026-10

95/100 stars

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Incubation:

Article Title: Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivation.
Article Snippet: NanoString spatial profiling was used for DSP analysis of three protein-level and 1800 transcriptomic-level immune-markers simultaneously on FFPE tissue slides45, 46. .. Following deparaffinization and antigen retrieval procedures, sections were simultaneously incubated overnight with fluorescent-labeled antibodies against CD3 (Origene), CD68 (Santa Cruz) and PanCytokeratin (Novus Biologicals), smooth muscle actin (Invitrogen), CD20 (Novus Biologicals), or cytokeratin 8/18 (Novus Biologicals) to visualize morphological features in the regions of interest (ROI). ..

Article Title: A Critical Role of Intracellular PD-L1 in Promoting Ovarian Cancer Progression
Article Snippet: Formalin-fixed paraffin-embedded (FFPE) ovarian tumor sections were deparaffinized at 65 °C for 1 h and then rehydrated through the use of xylene and an ethanol series; this was followed by antigen retrieval in sodium citrate buffer (Vector, Newark, CA, USA, #H-3300), pH 6.0, and signal enhancement via image-iTTM Fx-Signal Enhancer (Invitrogen, #I36933). .. Slides were then blocked with background-reducing components (Dako, Glostrup, Denmark, #S3022); this was followed by incubation with primary antibodies (PD-L1 (Cell Signaling Technology, #86744S), Ki67 (Vector, Newark, CA, USA, #VP-RM04), pancytokeratin (Novus, Chesterfield, MO, USA, #NBP2-33200AF647), E-cadherin (Cell Signaling Technology, #3195S), P21 (Santa Cruz Biotechnology, Dallas, TX, USA, #SC-6246), or γH2AX (Abcam, #ab26350)) at 4 °C overnight. .. The samples were then incubated with secondary antibodies using Biocare Medical Van Gogh Diluent (#PD902H) with either MACH 2 mouse (#MHRP520G) or MACH 2 rabbit (#RHRP520G) antibodies.

Article Title: A Critical Role of Intracellular PD-L1 in Promoting Ovarian Cancer Progression.
Article Snippet: Formalin-fixed paraffin-embedded (FFPE) ovarian tumor sections were deparaffinized at 65 ◦C for 1 h and then rehydrated through the use of xylene and an ethanol series; this was followed by antigen retrieval in sodium citrate buffer (Vector, Newark, CA, USA, #H-3300), pH 6.0, and signal enhancement via image-iTTM Fx-Signal Enhancer (Invitrogen, #I36933). .. Slides were then blocked with background-reducing components (Dako, Glostrup, Denmark, #S3022); this was followed by incubation with primary antibodies (PD-L1 (Cell Signaling Technology, #86744S), Ki67 (Vector, Newark, CA, USA, #VP-RM04), pancytokeratin (Novus, Chesterfield, MO, USA, #NBP2-33200AF647), E-cadherin (Cell Signaling Technology, #3195S), P21 (Santa Cruz Biotechnology, Dallas, TX, USA, #SC-6246), or γH2AX (Abcam, #ab26350)) at 4 ◦C overnight. .. The samples were then incubated with secondary antibodies using Biocare Medical Van Gogh Diluent (#PD902H) with either MACH 2 mouse (#MHRP520G) or MACH 2 rabbit (#RHRP520G) antibodies.

Labeling:

Article Title: B cells and energy metabolism in HER2-positive DCIS: insights into breast cancer progression from spatial-omics analyses
Article Snippet: .. To identify relevant regions of interest (ROIs), we used three fluorescently labeled morphology markers: pancytokeratin (PanCK, Novus #NBP2-33200DL594, clone AE1/AE3, dilution 1:400) to detect epithelial (cancer) cells; CD45 (Cell Signaling Technology #13917BF, clone D9M8I, dilution 1:100) to detect immune cells; and smooth muscle actin (SMA, Invitrogen, #53–5760-82, clone 1A4, dilution 1:400) to detect myoepithelial cells, endothelial cells and myofibroblasts, in addition to the nuclear marker Syto-83 (dilution 1:25). .. The slides were baked for 2 h at 60 °C and deparaffinized before being subjected to antigen retrieval for 20 min with BOND Epitope Retrieval 2 (Leica Microsystems, #AR9640) and proteinase K treatment at 0.1 μg/ml for 15 min.

Article Title: B cells and energy metabolism in HER2-positive DCIS: insights into breast cancer progression from spatial-omics analyses.
Article Snippet: .. To identify relevant regions of interest (ROIs), we used three fluorescently labeled morphology markers: pancytokeratin (PanCK, Novus #NBP2-33200DL594, clone AE1/AE3, dilution 1:400) to detect epithelial (cancer) cells; CD45 (Cell Signaling Technology #13917BF, clone D9M8I, dilution 1:100) to detect immune cells; and smooth muscle actin (SMA, Invitrogen, #53–5760-82, clone 1A4, dilution 1:400) to detect myoepithelial cells, endothelial cells and myofibroblasts, in addition to the nuclear marker Syto-83 (dilution 1:25). .. The slides were baked for 2 h at 60 °C and deparaffinized before being subjected to antigen retrieval for 20 min with BOND Epitope Retrieval 2 (Leica Microsystems, #AR9640) and proteinase K treatment at 0.1 μg/ml for 15 min.

Marker:

Staining:

Article Title: Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition
Article Snippet: mIHC staining was performed according to the manufacturer’s instructions (Opal TM 4-Color Anti-Rabbit Automation IHC; Opal TM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences ® ). .. The following primary rabbit antibodies were used for staining: CD4 ( CST25229 ; 1:200), CD8 ( CST98941 ; 1:400), F4/80 (CST 70076; 1:1000), and Ly6G (CST 87048; 1:1000) were purchased from Cell Signaling Technology ® ; CD11b ( Ab133357 ; 1:3000) from Abcam ® ; PanCytokeratin (NBP3-07280; 1:1000) from Novus Biologicals ® . .. Nuclei were stained with DAPI (Akoya Biosciences ® ).

Article Title: Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition
Article Snippet: mIHC staining was performed according to the manufacturer’s instructions (Opal TM 4-Color Anti-Rabbit Automation IHC; Opal TM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences ® ). .. The following primary rabbit antibodies were used for staining: CD4 (CST25229; 1:200), CD8 (CST98941; 1:400), F4/80 (CST 70076; 1:1000), and Ly6G (CST 87048; 1:1000) were purchased from Cell Signaling Technology ® ; CD11b (Ab133357; 1:3000) from Abcam ® ; PanCytokeratin (NBP3-07280; 1:1000) from Novus Biologicals ® . .. Nuclei were stained with DAPI (Akoya Biosciences ® ).

Article Title: Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition.
Article Snippet: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). .. The following primary rabbit antibodies were used for staining: CD4 (CST25229; 1:200), CD8 (CST98941; 1:400), F4/80 (CST 70076; 1:1000), and Ly6G (CST 87048; 1:1000) were purchased from Cell Signaling Technology®; CD11b (Ab133357; 1:3000) from Abcam®; PanCytokeratin (NBP3-07280; 1:1000) from Novus Biologicals®. .. Nuclei were stained with DAPI (Akoya Biosciences®).

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